Hotspot

rpoB-trnC-GCA

LSC · 26088-27348 · 1261 bp

Back to Brachycorythis

Pi 0.00641
MarkerSeek score 63.30
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1261
variable_sites
8
pi
0.00641
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 1397-1409 bp 1.0 75.6
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG CGACACCCAGATTCGAACTG 50.0 55.0 58.2 58.6 1421-1433 bp 1.0 74.5
rpoB-trnC-GCA_p3 AATTCTTCCGTCAAGCCCTG TTTGTTGATCAGGCGACACC 50.0 50.0 58.2 58.8 1434-1446 bp 1.0 73.6
rpoB-trnC-GCA_p4 AAATTCTTCCGTCAAGCCCT TAAAGGATTTGCAGTCCCCC 45.0 50.0 57.4 57.5 1398-1410 bp 1.0 72.2
rpoB-trnC-GCA_p5 AATTCTTCCGTCAAGCCCTG ATAAAGGATTTGCAGTCCCCC 50.0 47.6 58.2 58.0 1398-1410 bp 1.0 71.5