Hotspot

clpP

LSC · 66456-67106 · 651 bp

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Pi 0.0645

MarkerSeek score 76.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 42
pi: 0.0645
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GTTTAACCGTTGGCATGTCG	TGTTGTGTCAGAAAGCGTGA	50.0	45.0	58.0	58.0	1249 bp	1.0	85.7
clpP_p2	CACCCTAGCACATGTTCCTC	TGTTGTGTCAGAAAGCGTGA	55.0	45.0	58.0	58.0	1344 bp	1.0	85.5
clpP_p3	CCGTTGGCATGTCGTATGTA	TGTTGTGTCAGAAAGCGTGA	50.0	45.0	58.1	58.0	1243 bp	1.0	85.5
clpP_p4	CTGAACGAGTCGCACATACA	TGTTGTGTCAGAAAGCGTGA	50.0	45.0	58.0	58.0	1362 bp	1.0	85.4
clpP_p5	CCTAGCACATGTTCCTCGAC	TGTTGTGTCAGAAAGCGTGA	55.0	45.0	58.1	58.0	1341 bp	1.0	85.1