Hotspot

psaC-ndhE

SSC · 107244-107723 · 480 bp

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Pi 0.0387

MarkerSeek score 72.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 480
variable_sites: 18
pi: 0.0387
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTTGGACAAGCTCGTACACA	CTTCCATCCATCGTAACAGGA	50.0	47.6	58.1	57.2	610-617 bp	1.0	68.6
psaC-ndhE_p2	GTTGGACAAGCTCGTACACA	TCTTCCATCCATCGTAACAGG	50.0	47.6	58.1	57.2	611-618 bp	1.0	68.6
psaC-ndhE_p3	TGTTGGACAAGCTCGTACAC	CTTCCATCCATCGTAACAGGA	50.0	47.6	58.1	57.2	611-618 bp	1.0	68.6
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	TCTTCCATCCATCGTAACAGG	50.0	47.6	58.1	57.2	612-619 bp	1.0	68.6
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	CTTCCATCCATCGTAACAGGA	50.0	47.6	57.9	57.2	612-619 bp	1.0	68.5