Hotspot

ndhF-rpl32

SSC · 102467-103357 · 891 bp

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Pi 0.0398

MarkerSeek score 76.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 891
variable_sites: 29
pi: 0.0398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	836-974 bp	1.0	47.5
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	837-975 bp	1.0	47.5
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	838-976 bp	1.0	47.5
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	839-977 bp	1.0	47.5
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	840-978 bp	1.0	47.5