Hotspot

rpl33-rps18

LSC · 64036-64433 · 398 bp

Back to Bouteloua

Pi 0.121

MarkerSeek score 70.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 33
pi: 0.121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGTTCATGGCCAAGGGTAAA	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.1	58.0	846-934 bp	1.0	87.3
rpl33-rps18_p2	AAAGGAGGTTCATGGCCAAG	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.1	58.0	852-940 bp	1.0	87.3
rpl33-rps18_p3	GTACAATACACCCCGCCAAT	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.0	58.0	813 bp	0.5	59.6
rpl33-rps18_p4	GTACAATACACCCCGCCAAT	ACGAAAGGGTTGCTTGGATT	50.0	45.0	58.0	58.0	552 bp	0.5	59.6
rpl33-rps18_p5	GTACAATACACCCCGCCAAT	TTTCTGTGTCTAGGACCCGT	50.0	50.0	58.0	58.0	845 bp	0.5	59.6