Hotspot

psbE-petL

LSC · 60955-62109 · 1155 bp

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Pi 0.0391

MarkerSeek score 68.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 45
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	ACGCAGTCACGAATAATCCC	45.0	50.0	58.0	58.1	1620-1719 bp	1.0	81.9
psbE-petL_p2	TGACAAATAACCAACCCGCA	CAAAACCCGATGTCGTAGGT	45.0	50.0	58.0	57.9	1821-1924 bp	1.0	81.5
psbE-petL_p3	ATATTCGTTTGGCCGAGGAC	ACGCAGTCACGAATAATCCC	50.0	50.0	58.1	58.1	1670-1769 bp	1.0	81.5
psbE-petL_p4	CAAGTGGTCGTGCTAGAGAC	ACGCAGTCACGAATAATCCC	55.0	50.0	58.0	58.1	2125-2224 bp	1.0	81.4
psbE-petL_p5	CAAGTGGTCGTGCTAGAGAC	CAAAACCCGATGTCGTAGGT	55.0	50.0	58.0	57.9	2326-2429 bp	1.0	81.0