Hotspot

petN-trnC-GCA

LSC · 17665-18376 · 712 bp

Back to Bouteloua

Pi 0.0467

MarkerSeek score 76.90

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 33
pi: 0.0467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	761-1025 bp	1.0	73.9
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	763-1027 bp	1.0	73.4
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	CAGTTCAAATCTGGGTGCCG	50.0	55.0	58.2	59.5	762-1026 bp	1.0	70.5
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	AGGGGACTGCAAATCCTTTA	50.0	45.0	58.2	56.4	786-1050 bp	1.0	69.4
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTATCCCCAGT	50.0	42.9	58.2	57.2	779-1043 bp	1.0	68.2