Hotspot

trnD-GUC-psbM

LSC · 15767-16828 · 1062 bp

Back to Bouteloua

Pi 0.0378

MarkerSeek score 74.40

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 38
pi: 0.0378
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	GGAACTAGAATGAACAATGCAGT	50.0	39.1	58.6	57.1	1136-1178 bp	1.0	51.1
trnD-GUC-psbM_p2	TCAATTGGTTAGAGCACCGC	AGAATGAACAATGCAGTAGC	50.0	40.0	58.6	54.0	1130-1172 bp	1.0	50.1
trnD-GUC-psbM_p3	TCAATTGGTTAGAGCACCGC	ACTAGAATGAACAATGCAGTAGC	50.0	39.1	58.6	56.9	1133-1175 bp	1.0	49.7
trnD-GUC-psbM_p4	TCAATTGGTTAGAGCACCGC	AGGAACTAGAATGAACAATGCAG	50.0	39.1	58.6	56.9	1137-1179 bp	1.0	49.0
trnD-GUC-psbM_p5	TCAATTGGTTAGAGCACCGC	AGGAACTAGAATGAACAATGCA	50.0	36.4	58.6	55.7	1137-1179 bp	1.0	47.9