Hotspot

trnY-GUA-trnD-GUC

LSC · 15430-15692 · 263 bp

Back to Bouteloua

Pi 0.0532
MarkerSeek score 73.60
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
263
variable_sites
14
pi
0.0532
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 GGGGACGGACTGTAAATTCG GCGGTGCTCTAACCAATTGA 55.0 50.0 58.4 58.6 355-444 bp 1.0 75.6
trnY-GUA-trnD-GUC_p2 GGGACGGACTGTAAATTCGT GCGGTGCTCTAACCAATTGA 50.0 50.0 57.6 58.6 354-443 bp 1.0 75.4
trnY-GUA-trnD-GUC_p3 TGGGGACGGACTGTAAATTC GCGGTGCTCTAACCAATTGA 50.0 50.0 57.2 58.6 356-445 bp 1.0 72.2
trnY-GUA-trnD-GUC_p4 CTGGTTCAAATCCAGCTCGG GCGGTGCTCTAACCAATTGA 55.0 50.0 58.9 58.6 318-407 bp 1.0 71.4
trnY-GUA-trnD-GUC_p5 GGGGACGGACTGTAAATTCG GGCGGTGCTCTAACCAATT 55.0 52.6 58.4 58.1 356-445 bp 1.0 70.9