Hotspot

trnfM-CAU-trnG-UCC

LSC · 12733-12822 · 90 bp

Back to Bouteloua

Pi 0.0556
MarkerSeek score 73.10
Primer available yes
Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name
trnfM-CAU-trnG-UCC
feature_type
igs
region
LSC
length_bp
90
variable_sites
5
pi
0.0556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-UCC_p1 CCTCAAGGTTATGAGCCTCG AGCCTTCCAAGCTAACGATG 55.0 50.0 57.8 58.0 181-190 bp 1.0 80.1
trnfM-CAU-trnG-UCC_p2 CCTCAAGGTTATGAGCCTCG CCTAGCCTTCCAAGCTAACG 55.0 55.0 57.8 58.1 184-193 bp 1.0 79.8
trnfM-CAU-trnG-UCC_p3 CCTCGTGAGCTACCAAACTG AGCCTTCCAAGCTAACGATG 55.0 50.0 58.3 58.0 166-175 bp 1.0 79.8
trnfM-CAU-trnG-UCC_p4 CCTCGTGAGCTACCAAACTG CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.3 58.1 169-178 bp 1.0 79.6
trnfM-CAU-trnG-UCC_p5 CCTCAAGGTTATGAGCCTCG CCAACGTCGACTATAACCCC 55.0 55.0 57.8 57.8 202-211 bp 1.0 78.4