Hotspot

trnS-UGA-psbZ

LSC · 11590-11930 · 341 bp

Back to Bouteloua

Pi 0.0205

MarkerSeek score 51.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 7
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CATTTGTGGCATGCAGGAAG	TGGGTCGACCGTTTCATTTT	50.0	45.0	58.0	58.0	958-962 bp	1.0	85.2
trnS-UGA-psbZ_p2	CATTTGTGGCATGCAGGAAG	CTCCGCACTTAGCAGTTCAT	50.0	50.0	58.0	58.0	1415 bp	0.5	59.8
trnS-UGA-psbZ_p3	AGCGTCTTGGAGCTAATGTG	CTCCGCACTTAGCAGTTCAT	50.0	50.0	58.0	58.0	1771 bp	0.5	59.7
trnS-UGA-psbZ_p4	CAGCGTCTTGGAGCTAATGT	CTCCGCACTTAGCAGTTCAT	50.0	50.0	58.0	58.0	1772 bp	0.5	59.7
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	CTCCGCACTTAGCAGTTCAT	55.0	50.0	58.0	58.0	1098 bp	0.5	59.7