Hotspot

psbC-trnS-UGA

LSC · 11354-11501 · 148 bp

Back to Bouteloua

Pi 0.164

MarkerSeek score 79.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 23
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GTTCTTTACATGACTCCTCTT	GGGTTCGAATCCCTCTCTCT	38.1	55.0	52.9	58.2	198-212 bp	1.0	51.1
psbC-trnS-UGA_p2	GTTCTTTACATGACTCCTCTT	ATAGCCCCGGTCTTGAAAAC	38.1	50.0	52.9	57.9	242-256 bp	1.0	50.0
psbC-trnS-UGA_p3	GTTCTTTACATGACTCCTCTT	ATGGCTGAGTGGTTGATAGC	38.1	50.0	52.9	57.7	257-271 bp	1.0	49.4
psbC-trnS-UGA_p4	GTTCTTTACATGACTCCTCTT	AGAGATGGCTGAGTGGTTGA	38.1	50.0	52.9	58.4	261-275 bp	1.0	49.3
psbC-trnS-UGA_p5	GTTCTTTACATGACTCCTCTT	GAGAGATGGCTGAGTGGTTG	38.1	55.0	52.9	58.0	262-276 bp	1.0	49.2