Hotspot

rpl32-trnL-UAG

SSC · 114616-115509 · 894 bp

Back to Bougainvillea

Pi 0.0142

MarkerSeek score 63.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 894
variable_sites: 45
pi: 0.0142
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAAAGGACGGTTGGTAGC	TCCTAAGAGCAGCGTGTCTA	52.6	50.0	56.2	57.9	981-1031 bp	1.0	64.5
rpl32-trnL-UAG_p2	GAAAAGGACGGTTGGTAGC	AGCAGCGTGTCTACCAATTT	52.6	45.0	56.2	57.8	974-1024 bp	1.0	64.0
rpl32-trnL-UAG_p3	GAAAAGGACGGTTGGTAGC	GCTCTTGCACTGCTTCCTAA	52.6	50.0	56.2	58.2	995-1045 bp	1.0	64.0
rpl32-trnL-UAG_p4	GAAAAGGACGGTTGGTAGC	TTGCACTGCTTCCTAAGAGC	52.6	50.0	56.2	58.2	991-1041 bp	1.0	64.0
rpl32-trnL-UAG_p5	GAAAAGGACGGTTGGTAGC	CTAAGAGCAGCGTGTCTACC	52.6	55.0	56.2	57.8	979-1029 bp	1.0	63.9