Hotspot

ndhF-rpl32

SSC · 113591-114456 · 866 bp

Back to Bougainvillea

Pi 0.015

MarkerSeek score 61.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 866
variable_sites: 61
pi: 0.015
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCATGCATATTGGTATATATGTTCCA	AGAAAGAGAGGTACGTTTTT	34.6	35.0	57.2	52.1	921-979 bp	1.0	49.1
ndhF-rpl32_p2	TCCATGCATATTGGTATATATGTTCC	AGAAAGAGAGGTACGTTTTT	34.6	35.0	57.0	52.1	922-980 bp	1.0	49.1
ndhF-rpl32_p3	GGTATAATCCATGCATATTGGT	AGAAAGAGAGGTACGTTTTT	36.4	35.0	53.8	52.1	929-987 bp	1.0	49.0
ndhF-rpl32_p4	GGTATAATCCATGCATATTGGT	TAGAAAGAGAGGTACGTTTTT	36.4	33.3	53.8	52.2	930-988 bp	1.0	49.0
ndhF-rpl32_p5	GGTATAATCCATGCATATTGGT	TTAGAAAGAGAGGTACGTTTT	36.4	33.3	53.8	52.2	931-989 bp	1.0	49.0