Hotspot

rps18-rpl20

LSC · 70093-70327 · 235 bp

Back to Bougainvillea

Pi 0.0168

MarkerSeek score 64.50

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 235
variable_sites: 14
pi: 0.0168
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TCGAGTCGACTGCTAGAACT	GGAAAGAATCCCCCGAAATT	50.0	45.0	57.9	55.7	274-314 bp	1.0	69.0
rps18-rpl20_p2	CGAGTCGACTGCTAGAACTG	GGAAAGAATCCCCCGAAATT	55.0	45.0	57.8	55.7	273-313 bp	1.0	68.4
rps18-rpl20_p3	TCGAGTCGACTGCTAGAACT	GGAAAGAATCCCCCGAAATTT	50.0	42.9	57.9	56.5	314 bp	0.066667	21.6
rps18-rpl20_p4	CGAGTCGACTGCTAGAACTG	GGAAAGAATCCCCCGAAATTT	55.0	42.9	57.8	56.5	313 bp	0.066667	21.0
rps18-rpl20_p5	TCGAGTCGACTGCTAGAACT	GGAAAGAATCCCCCGAAATTTT	50.0	40.9	57.9	57.2	314 bp	0.066667	19.3