Hotspot

ycf3-trnS-GGA

LSC · 45340-46166 · 827 bp

Back to Bougainvillea

Pi 0.0122

MarkerSeek score 66.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 34
pi: 0.0122
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCCATTTATCCGCGATCTCG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.7	58.1	896-955 bp	1.0	83.4
ycf3-trnS-GGA_p2	TCCATTTATCCGCGATCTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.7	58.2	917-976 bp	1.0	82.9
ycf3-trnS-GGA_p3	TCCATTTATCCGCGATCTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.7	57.8	867-926 bp	1.0	82.7
ycf3-trnS-GGA_p4	TCCATTTATCCGCGATCTCG	ACCCTCGGTAAACAAAAGCC	50.0	50.0	57.7	58.4	897-956 bp	1.0	81.1
ycf3-trnS-GGA_p5	TCCATTTATCCGCGATCTCG	AGTTCCAATGCTACGCCTTG	50.0	50.0	57.7	58.5	869-928 bp	1.0	79.9