Hotspot

psbM-trnD-GUC

LSC · 29952-30945 · 994 bp

Back to Bougainvillea

Pi 0.0158

MarkerSeek score 68.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 994
variable_sites: 42
pi: 0.0158
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TACCTCAGTTTCCGGGGATT	45.0	50.0	55.2	58.0	1092-1135 bp	1.0	64.2
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TACCTCAGTTTCCGGGGATT	43.5	50.0	58.0	58.0	1095-1138 bp	1.0	62.7
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TACCTCAGTTTCCGGGGATT	43.5	50.0	57.9	58.0	1099-1142 bp	1.0	62.0
psbM-trnD-GUC_p4	AGAATGAAGAGTGCAGTAGC	ATACCTCAGTTTCCGGGGAT	45.0	50.0	55.2	57.8	1093-1136 bp	0.933333	59.6
psbM-trnD-GUC_p5	ACTAGAATGAAGAGTGCAGTAGC	ATACCTCAGTTTCCGGGGAT	43.5	50.0	58.0	57.8	1096-1139 bp	0.933333	58.1