Hotspot

petN-psbM

LSC · 28715-29846 · 1132 bp

Back to Bougainvillea

Pi 0.00364

MarkerSeek score 63.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 19
pi: 0.00364
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	1262-1299 bp	1.0	68.2
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	1208-1245 bp	1.0	65.5
petN-psbM_p3	TGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.1	57.0	1268-1305 bp	0.933333	64.7
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	1262-1299 bp	1.0	63.8
petN-psbM_p5	TGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTC	50.0	45.0	58.1	55.5	1268-1305 bp	0.933333	60.3