Hotspot

rps16-trnQ-UUG

LSC · 6245-6998 · 754 bp

Back to Bougainvillea

Pi 0.023

MarkerSeek score 72.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 61
pi: 0.023
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	842-887 bp	1.0	88.1
rps16-trnQ-UUG_p2	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	816-861 bp	1.0	87.6
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	832-877 bp	1.0	87.5
rps16-trnQ-UUG_p4	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	872-917 bp	1.0	87.2
rps16-trnQ-UUG_p5	GCACGTTGCTTTTTACCACA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	57.8	58.2	846-891 bp	1.0	86.7