Hotspot

rpl32

SSC · 124085-124246 · 162 bp

Back to Bothriospermum

Pi 0.444

MarkerSeek score 77.20

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 162
variable_sites: 68
pi: 0.444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	CTACAGGGTGAAGCGAGTTC	CAAGGGTATGATCCACGCAT	55.0	50.0	58.0	57.7	1017 bp	0.5	57.9
rpl32_p2	CTACAGGGTGAAGCGAGTTC	AAGCAAGGGTATGATCCACG	55.0	50.0	58.0	57.7	1020 bp	0.5	57.3
rpl32_p3	TAGCGGCTTGTCTTCAACTC	CAAGGGTATGATCCACGCAT	50.0	50.0	57.9	57.7	1489 bp	0.5	57.3
rpl32_p4	TCCTAAGAGCAGCGTGTCTA	CAAGGGTATGATCCACGCAT	50.0	50.0	57.9	57.7	1521 bp	0.5	56.9
rpl32_p5	ATTTCACCATAGCGGCTTGT	CAAGGGTATGATCCACGCAT	45.0	50.0	57.9	57.7	1498 bp	0.5	56.9