Hotspot

ndhE

SSC · 119759-120064 · 306 bp

Back to Bothriospermum

Pi 0.496

MarkerSeek score 75.40

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 133
pi: 0.496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TTTCATCTTTGGACCGTGGG	GGGAGCCAGTGAATATTGGG	50.0	55.0	58.1	58.0	1522 bp	0.5	59.1
ndhE_p2	TTTCATCTTTGGACCGTGGG	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.1	58.0	1190 bp	0.5	59.1
ndhE_p3	TTTCATCTTTGGACCGTGGG	AAACCCGTGGTCGAAAATCA	50.0	45.0	58.1	58.0	1385 bp	0.5	59.0
ndhE_p4	TTTCATCTTTGGACCGTGGG	CAGTCCTCTGTTCTTGGAGC	50.0	55.0	58.1	57.9	1193 bp	0.5	58.5
ndhE_p5	TTTCATCTTTGGACCGTGGG	GTCCTCTGTTCTTGGAGCAG	50.0	55.0	58.1	57.9	1191 bp	0.5	58.5