Hotspot

ndhA

SSC · 115925-118090 · 2166 bp

Back to Bothriospermum

Pi 0.476

MarkerSeek score 77.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2166
variable_sites: 808
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GAAACCCGGCGTTTTGTTAG	GGTAACCGACCTAACGCAAT	50.0	50.0	58.0	58.0	2988 bp	0.5	59.6
ndhA_p2	AGCCCCAAAAGGAGAATTGG	GGTAACCGACCTAACGCAAT	50.0	50.0	58.1	58.0	2866 bp	0.5	59.4
ndhA_p3	GCGTAGGCATTATTGGTGGA	GCGTGGGACAAGGTAATCAT	50.0	50.0	58.0	58.0	2906 bp	0.5	59.4
ndhA_p4	GAAACCCGGCGTTTTGTTAG	GCGTGGGACAAGGTAATCAT	50.0	50.0	58.0	58.0	2649 bp	0.5	59.4
ndhA_p5	AGCCCCAAAAGGAGAATTGG	GCGTGGGACAAGGTAATCAT	50.0	50.0	58.1	58.0	2527 bp	0.5	59.2