Hotspot

ccsA

SSC · 122402-123292 · 891 bp

Back to Bothriospermum

Pi 0.502

MarkerSeek score 81.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 891
variable_sites: 386
pi: 0.502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GATTCTGGACCGCGAGAATT	GAACTCGCTTCACCCTGTAG	50.0	55.0	58.1	58.0	1866 bp	0.5	59.5
ccsA_p2	TTTTTGATTCTGGACCGCGA	GAACTCGCTTCACCCTGTAG	45.0	55.0	58.1	58.0	1871 bp	0.5	59.1
ccsA_p3	GATTCTGGACCGCGAGAATT	GAGTTGAAGACAAGCCGCTA	50.0	50.0	58.1	57.9	1394 bp	0.5	58.9
ccsA_p4	TAGCTTTTCCATGGCTTCCC	GAACTCGCTTCACCCTGTAG	50.0	55.0	57.9	58.0	2095 bp	0.5	58.8
ccsA_p5	GATTCTGGACCGCGAGAATT	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.1	57.9	1362 bp	0.5	58.5