Hotspot

rpl32-ndhF

SSC · 124247-124917 · 671 bp

Back to Bothriospermum

Pi 0.454

MarkerSeek score 79.00

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 671
variable_sites: 240
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CTACAGGGTGAAGCGAGTTC	TCGGAAAAAGAAGAAGCCCC	55.0	50.0	58.0	58.1	1069 bp	0.5	59.2
rpl32-ndhF_p2	TGCCGCACAACAAAACTTTT	TCGGAAAAAGAAGAAGCCCC	40.0	50.0	57.9	58.1	911 bp	0.5	58.6
rpl32-ndhF_p3	TTGCCGCACAACAAAACTTT	TCGGAAAAAGAAGAAGCCCC	40.0	50.0	57.9	58.1	912 bp	0.5	58.6
rpl32-ndhF_p4	TTTGCCGCACAACAAAACTT	TCGGAAAAAGAAGAAGCCCC	40.0	50.0	57.9	58.1	913 bp	0.5	58.6
rpl32-ndhF_p5	CTGTCCAATATCCCCTCCCT	TCGGAAAAAGAAGAAGCCCC	55.0	50.0	57.9	58.1	844 bp	0.5	58.2