Hotspot

trnL-UAG-rpl32

SSC · 123475-124084 · 610 bp

Back to Bothriospermum

Pi 0.411

MarkerSeek score 81.80

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 610
variable_sites: 222
pi: 0.411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	AAAAGTTTTGTTGTGCGGCA	50.0	40.0	57.9	57.9	682 bp	0.5	58.3
trnL-UAG-rpl32_p2	TCCTAAGAGCAGCGTGTCTA	AAAGTTTTGTTGTGCGGCAA	50.0	40.0	57.9	57.9	681 bp	0.5	58.3
trnL-UAG-rpl32_p3	TCCTAAGAGCAGCGTGTCTA	AAGTTTTGTTGTGCGGCAAA	50.0	40.0	57.9	57.9	680 bp	0.5	58.3
trnL-UAG-rpl32_p4	TCCTAAGAGCAGCGTGTCTA	AGGGAGGGGATATTGGACAG	50.0	55.0	57.9	57.9	749 bp	0.5	58.0
trnL-UAG-rpl32_p5	AGCAGCGTGTCTACCAATTT	AAGTTTTGTTGTGCGGCAAA	45.0	40.0	57.8	57.9	673 bp	0.5	57.8