Hotspot

ccsA-trnL-UAG

SSC · 123293-123394 · 102 bp

Back to Bothriospermum

Pi 0.4

MarkerSeek score 76.80

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 38
pi: 0.4
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	CCGAAAGAATCCCCACAGTT	GAACTCGCTTCACCCTGTAG	50.0	55.0	57.8	58.0	1310 bp	0.5	58.4
ccsA-trnL-UAG_p2	GCCCCCAATAAATCCAACGA	GAACTCGCTTCACCCTGTAG	50.0	55.0	58.2	58.0	860 bp	0.5	58.1
ccsA-trnL-UAG_p3	CTTAGTGGAAAATGCCCCCA	GAACTCGCTTCACCCTGTAG	50.0	55.0	57.8	58.0	873 bp	0.5	58.1
ccsA-trnL-UAG_p4	ACTTAGTGGAAAATGCCCCC	GAACTCGCTTCACCCTGTAG	50.0	55.0	57.8	58.0	874 bp	0.5	58.1
ccsA-trnL-UAG_p5	CCAAGTTTCCTTCGGATCCC	GAACTCGCTTCACCCTGTAG	55.0	55.0	58.2	58.0	1372 bp	0.5	58.0