Hotspot

ndhD-ccsA

SSC · 122232-122401 · 170 bp

Back to Bothriospermum

Pi 0.461

MarkerSeek score 75.80

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 170
variable_sites: 70
pi: 0.461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	GAGCATATGGGTTGGTTCGT	GGGCTACACAGTTACGGTTT	50.0	50.0	58.0	57.8	946 bp	0.5	58.3
ndhD-ccsA_p2	GAGCATATGGGTTGGTTCGT	GCAATTGTGGCGTCAATAGG	50.0	50.0	58.0	57.8	1015 bp	0.5	58.1
ndhD-ccsA_p3	GATTCTGGACCGCGAGAATT	GGGCTACACAGTTACGGTTT	50.0	50.0	58.1	57.8	350 bp	0.5	58.1
ndhD-ccsA_p4	GAGCATATGGGTTGGTTCGT	AACTGTGGGGATTCTTTCGG	50.0	50.0	58.0	57.8	1172 bp	0.5	58.1
ndhD-ccsA_p5	GAGCATATGGGTTGGTTCGT	TGCAAATTCCGCAATTGTGG	50.0	45.0	58.0	58.2	1025 bp	0.5	58.0