Hotspot

rps15-ndhH

SSC · 114642-114741 · 100 bp

Back to Bothriospermum

Pi 0.426

MarkerSeek score 77.10

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 100
variable_sites: 40
pi: 0.426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	TTGGGAAAACGTCAACGACT	GGTCTGTGCCCCAATATCTG	45.0	55.0	58.0	58.0	592 bp	0.5	59.6
rps15-ndhH_p2	TTGGGAAAACGTCAACGACT	CACCAATAATGCCTACGCCT	45.0	50.0	58.0	58.0	836 bp	0.5	59.5
rps15-ndhH_p3	TTGGGAAAACGTCAACGACT	TCCACCAATAATGCCTACGC	45.0	50.0	58.0	58.0	838 bp	0.5	59.5
rps15-ndhH_p4	TTGGGAAAACGTCAACGACT	TCCTAACTGTTCGGGTCCAT	45.0	50.0	58.0	58.1	475 bp	0.5	59.4
rps15-ndhH_p5	TTGGGAAAACGTCAACGACT	AACACCATGCATTGATGGGT	45.0	45.0	58.0	58.1	280 bp	0.5	59.3