Hotspot

ycf4

LSC · 56741-57298 · 558 bp

Back to Bothriochloa

Pi 0.00358

MarkerSeek score 59.80

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 558
variable_sites: 3
pi: 0.00358
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TTCAATTGACCGCTACTGGA	TCCTCGTTTGTTCCTTCCTT	45.0	45.0	56.9	56.7	665-670 bp	1.0	60.7
ycf4_p2	TCAATTGACCGCTACTGGAT	TCCTCGTTTGTTCCTTCCTT	45.0	45.0	56.6	56.7	664-669 bp	1.0	58.9
ycf4_p3	TTCAATTGACCGCTACTGGA	ATCCTCGTTTGTTCCTTCCT	45.0	45.0	56.9	56.5	666-671 bp	1.0	58.8
ycf4_p4	TTCAATTGACCGCTACTGGA	TCCTCGTTTGTTCCTTCCTTT	45.0	42.9	56.9	57.4	665-670 bp	1.0	58.7
ycf4_p5	TTTCAATTGACCGCTACTGGA	TCCTCGTTTGTTCCTTCCTT	42.9	45.0	57.6	56.7	666-671 bp	1.0	58.5