Hotspot

petA

LSC · 58592-59554 · 963 bp

Back to Bothriochloa

Pi 0.000692

MarkerSeek score 45.10

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.000692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	GTAGAAATTCCGGGATCCGC	GTTCTCGTCTTTGCCTTGGA	55.0	50.0	58.5	58.1	1094 bp	1.0	75.2
petA_p2	GTAGAAATTCCGGGATCCGC	TCTCGTCTTTGCCTTGGATC	55.0	50.0	58.5	57.6	1092 bp	1.0	73.1
petA_p3	GTAGAAATTCCGGGATCCGC	TGTTCTCGTCTTTGCCTTGG	55.0	50.0	58.5	58.4	1095 bp	1.0	72.9
petA_p4	GTAGAAATTCCGGGATCCGC	CGTCTTTGCCTTGGATCCTC	55.0	55.0	58.5	58.6	1089 bp	1.0	71.1
petA_p5	GTAGAAATTCCGGGATCCGC	CTCGTCTTTGCCTTGGATCC	55.0	55.0	58.5	58.6	1091 bp	1.0	71.1