Hotspot

petG-trnW-CCA

LSC · 62866-62984 · 119 bp

Back to Bothriochloa

Pi 0.0112

MarkerSeek score 59.60

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 2
pi: 0.0112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	216 bp	1.0	79.5
petG-trnW-CCA_p2	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	219 bp	1.0	78.2
petG-trnW-CCA_p3	GCGTATTTGCAATACAGGCG	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.3	57.9	200 bp	1.0	78.0
petG-trnW-CCA_p4	GGGATTATTCGTGACTGCGT	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.1	57.6	211 bp	1.0	77.3
petG-trnW-CCA_p5	GGGATTATTCGTGACTGCGT	TCCAAAACCCGATGTCGTAG	50.0	50.0	58.1	57.6	218 bp	1.0	77.2