Hotspot

rbcL-psaI

LSC · 55339-56264 · 926 bp

Back to Bothriochloa

Pi 0.00515

MarkerSeek score 50.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 7
pi: 0.00515
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	1594-1623 bp	1.0	81.6
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1808-1837 bp	1.0	81.3
rbcL-psaI_p3	TCTCATTATTGCCGCGACAA	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	2098-2127 bp	1.0	81.1
rbcL-psaI_p4	TTGTCTCATTATTGCCGCGA	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	2101-2130 bp	1.0	81.1
rbcL-psaI_p5	GGCTTCAGGGGGTATTCATG	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.0	57.9	1279-1298 bp	1.0	81.0