Hotspot

atpB-rbcL

LSC · 53102-53907 · 806 bp

Back to Bothriochloa

Pi 0.00166

MarkerSeek score 61.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 2
pi: 0.00166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	980-988 bp	1.0	78.1
atpB-rbcL_p2	TATCCAGCACGGGTCCAATA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	979-987 bp	1.0	78.1
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	977-985 bp	1.0	77.8
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	976-984 bp	1.0	77.8
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	ACTCCGGGGTGTAGTAAGTC	55.0	55.0	57.7	58.2	995-1003 bp	1.0	77.5