Hotspot

trnL-UAA-trnF-GAA

LSC · 46481-46848 · 368 bp

Back to Bothriochloa

Pi 0.00906

MarkerSeek score 63.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 5
pi: 0.00906
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	448-486 bp	1.0	78.1
trnL-UAA-trnF-GAA_p2	AAGTCGTGAGGGTTCAAGTC	CTGTGCCAGGAACCAGATTT	50.0	50.0	57.5	58.1	465-503 bp	1.0	77.8
trnL-UAA-trnF-GAA_p3	AAGTCGTGAGGGTTCAAGTC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	463-501 bp	1.0	77.7
trnL-UAA-trnF-GAA_p4	AAGTCGTGAGGGTTCAAGTC	TTTTTCTGTGCCAGGAACCA	50.0	45.0	57.5	57.8	470-508 bp	1.0	77.2
trnL-UAA-trnF-GAA_p5	AAGTCGTGAGGGTTCAAGTC	TTTTCTGTGCCAGGAACCAG	50.0	50.0	57.5	58.3	469-507 bp	1.0	75.9