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atpI-atpH

LSC · 30714-31531 · 818 bp

Back to Bothriochloa

Pi 0.000815

MarkerSeek score 59.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 1
pi: 0.000815
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CAGTACCTTGACCAACTCCG	50.0	55.0	57.5	57.9	952-956 bp	1.0	75.3
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CAGTACCTTGACCAACTCCG	45.0	55.0	58.6	57.9	965-969 bp	1.0	74.8
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	953-957 bp	1.0	74.4
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	886-890 bp	1.0	74.3
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	908-912 bp	1.0	74.2