Hotspot

petN-rpoB

LSC · 16865-18616 · 1752 bp

Back to Bothriochloa

Pi 0.00609

MarkerSeek score 50.50

Primer available yes

Label petN-rpoB

Feature Metadata

label_name: petN-rpoB
feature_type: igs
region: LSC
length_bp: 1752
variable_sites: 16
pi: 0.00609
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-rpoB_p1	AGCCCAAGCGAGACTTACTA	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.2	58.4	1803-1816 bp	1.0	76.6
petN-rpoB_p2	AGCCCAAGCGAGACTTACTA	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.2	57.6	1804-1817 bp	1.0	76.4
petN-rpoB_p3	CCACTCCTCCCCCATACTAC	TCCCTCATTTCCATTCCGGA	60.0	50.0	58.3	58.4	1859-1872 bp	1.0	75.5
petN-rpoB_p4	CCACTCCTCCCCCATACTAC	TTCCCTCATTTCCATTCCGG	60.0	50.0	58.3	57.6	1860-1873 bp	1.0	75.3
petN-rpoB_p5	TCCACTCCTCCCCCATACTA	TCCCTCATTTCCATTCCGGA	55.0	50.0	58.4	58.4	1860-1873 bp	1.0	74.8