Hotspot

trnG-UCC-trnM-CAU

LSC · 13217-13946 · 730 bp

Back to Bothriochloa

Pi 0.00365

MarkerSeek score 62.50

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 4
pi: 0.00365
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	TCGAACCGATGACTTCTGTC	38.1	50.0	55.5	57.4	874-888 bp	1.0	47.8
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	CGAACCGATGACTTCTGTCT	38.1	50.0	55.5	57.4	873-887 bp	1.0	47.8
trnG-UCC-trnM-CAU_p3	AGAACGAATCACACTTTTACCAC	TCGAACCGATGACTTCTGTC	39.1	50.0	57.3	57.4	877-891 bp	1.0	46.8
trnG-UCC-trnM-CAU_p4	GAACGAATCACACTTTTACCACT	TCGAACCGATGACTTCTGTC	39.1	50.0	57.3	57.4	876-890 bp	1.0	46.8
trnG-UCC-trnM-CAU_p5	GAACGAATCACACTTTTACCACT	CGAACCGATGACTTCTGTCT	39.1	50.0	57.3	57.4	875-889 bp	1.0	46.7