Hotspot

clpP

LSC · 72930-74991 · 2062 bp

Back to Boswellia

Pi 0.00557

MarkerSeek score 47.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2062
variable_sites: 28
pi: 0.00557
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGTGTATTTGCGTGAAATCG	ACGCTTCCACATCAAAGTGA	42.9	45.0	57.6	57.7	2151-2159 bp	1.0	68.3
clpP_p2	TGGAAATCGTGTATTTGCGTG	ACGCTTCCACATCAAAGTGA	42.9	45.0	57.5	57.7	2157-2165 bp	1.0	67.5
clpP_p3	GGAAATCGTGTATTTGCGTGA	ACGCTTCCACATCAAAGTGA	42.9	45.0	57.3	57.7	2156-2164 bp	1.0	65.4
clpP_p4	CGTGTATTTGCGTGAAATCG	ACGCTTCCACATCAAAGTGA	45.0	45.0	56.2	57.7	2150-2158 bp	1.0	64.6
clpP_p5	TGGAAATCGTGTATTTGCGT	ACGCTTCCACATCAAAGTGA	40.0	45.0	56.1	57.7	2157-2165 bp	1.0	63.7