Hotspot

trnN-GUU-trnR-ACG

IRa · 133799-134406 · 608 bp

Back to Boswellia

Pi 0.00424

MarkerSeek score 57.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 608
variable_sites: 6
pi: 0.00424
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	ATGAGATAGAACCTCCCGCA	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.9	58.1	813-815 bp	1.0	79.1
trnN-GUU-trnR-ACG_p2	TGGGTTGAGAACGGGAATTG	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.1	58.1	839-841 bp	1.0	79.0
trnN-GUU-trnR-ACG_p3	AGATAGAACCTCCCGCAGTT	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.1	58.1	810-812 bp	1.0	78.8
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	762-764 bp	1.0	78.7
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.2	58.1	749-751 bp	1.0	78.7