Hotspot

trnR-ACG-trnN-GUU

IRb · 111653-112260 · 608 bp

Back to Boswellia

Pi 0.00424

MarkerSeek score 57.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 608
variable_sites: 6
pi: 0.00424
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	TAGGGTACTTCCGGGAACAG	ATGAGATAGAACCTCCCGCA	55.0	50.0	58.1	57.9	813-815 bp	1.0	79.1
trnR-ACG-trnN-GUU_p2	TAGGGTACTTCCGGGAACAG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.1	58.1	839-841 bp	1.0	79.0
trnR-ACG-trnN-GUU_p3	TAGGGTACTTCCGGGAACAG	AGATAGAACCTCCCGCAGTT	55.0	50.0	58.1	58.1	810-812 bp	1.0	78.8
trnR-ACG-trnN-GUU_p4	TAGGGTACTTCCGGGAACAG	GCTGTTAACTGACTGGTCGT	55.0	50.0	58.1	57.8	762-764 bp	1.0	78.7
trnR-ACG-trnN-GUU_p5	TAGGGTACTTCCGGGAACAG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.1	58.2	749-751 bp	1.0	78.7