Hotspot

clpP-psbB

LSC · 74992-75429 · 438 bp

Back to Boswellia

Pi 0.00594

MarkerSeek score 57.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 7
pi: 0.00594
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	509-534 bp	1.0	83.1
clpP-psbB_p2	CGCACTATACGTCAACCCAA	CAAACGACCGGGATCATTCA	50.0	50.0	58.0	58.3	546-571 bp	1.0	82.8
clpP-psbB_p3	GACAGGTCGCACTATACGTC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.9	58.2	516-541 bp	1.0	82.3
clpP-psbB_p4	CTGACAGGTCGCACTATACG	CGATACCAAGGCAAACCCAT	55.0	50.0	57.9	58.2	518-543 bp	1.0	82.3
clpP-psbB_p5	GACAGGTCGCACTATACGTC	CAAACGACCGGGATCATTCA	55.0	50.0	57.9	58.3	553-578 bp	1.0	82.0