Hotspot

trnF-GAA-ndhJ

LSC · 50959-51532 · 574 bp

Back to Boswellia

Pi 0.016

MarkerSeek score 71.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 574
variable_sites: 28
pi: 0.016
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGGCTGAAACGTATC	55.0	50.0	58.0	58.1	617-748 bp	1.0	86.5
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	585-716 bp	1.0	86.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCACGGCTGAAACGTATC	50.0	50.0	58.1	58.1	581-712 bp	1.0	86.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	549-680 bp	1.0	86.3
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	ATCCACGGCTGAAACGTATC	55.0	50.0	57.9	58.1	605-736 bp	1.0	85.5