Hotspot

trnC-GCA-petN

LSC · 29457-30199 · 743 bp

Back to Boswellia

Pi 0.00514

MarkerSeek score 62.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 12
pi: 0.00514
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ACAAAACAAGCAAAGGGGGA	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.1	58.2	850-888 bp	1.0	82.3
trnC-GCA-petN_p2	TTGTATCCTTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.2	58.2	815-853 bp	1.0	82.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	779-817 bp	1.0	80.9
trnC-GCA-petN_p4	ACAAAACAAGCAAAGGGGGA	AGAGTCCACTTCTTCCCCAT	45.0	50.0	58.1	57.6	911-949 bp	1.0	80.4
trnC-GCA-petN_p5	TTGTATCCTTTTGGCGGCAT	AGAGTCCACTTCTTCCCCAT	45.0	50.0	58.2	57.6	876-914 bp	1.0	80.4