Hotspot

atpF-atpH

LSC · 14488-15003 · 516 bp

Back to Boswellia

Pi 0.0111

MarkerSeek score 64.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 20
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGAACCAAGGAAACGA	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	58.0	57.5	643-693 bp	1.0	81.9
atpF-atpH_p2	CAAATGGCCAGTGAACCAAG	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.6	57.5	650-700 bp	1.0	78.6
atpF-atpH_p3	CCAGTGAACCAAGGAAACGA	TGGACTGGTTGTAGCATTAGC	50.0	47.6	58.0	58.0	572-622 bp	1.0	78.2
atpF-atpH_p4	CCAGTGAACCAAGGAAACGA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.0	58.0	637-687 bp	1.0	78.0
atpF-atpH_p5	CCAGTGAACCAAGGAAACGA	TGAGGCAGAGGGAAAAATACG	50.0	47.6	58.0	58.0	641-691 bp	1.0	78.0