Hotspot

trnS-GCU-trnG-UCC

LSC · 9462-10407 · 946 bp

Back to Boswellia

Pi 0.00823

MarkerSeek score 57.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 946
variable_sites: 24
pi: 0.00823
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	AACTGAAGGACCCCTTAACT	50.0	45.0	58.0	55.6	891-1077 bp	1.0	67.0
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AACTGAAGGACCCCTTAACT	50.0	45.0	58.2	55.6	949-1135 bp	1.0	65.7
trnS-GCU-trnG-UCC_p3	CCGACGCTTTAATCCACTCA	AAACTGAAGGACCCCTTAACT	50.0	42.9	58.0	56.5	892-1078 bp	1.0	65.5
trnS-GCU-trnG-UCC_p4	ACGCTTTAATCCACTCAGCC	AACTGAAGGACCCCTTAACT	50.0	45.0	58.3	55.6	888-1074 bp	1.0	65.0
trnS-GCU-trnG-UCC_p5	CGCTTTAATCCACTCAGCCA	AACTGAAGGACCCCTTAACT	50.0	45.0	58.3	55.6	887-1073 bp	1.0	64.9