Hotspot

trnK-UUU-rps16

LSC · 4329-5384 · 1056 bp

Back to Boswellia

Pi 0.0245

MarkerSeek score 66.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 88
pi: 0.0245
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GTCTTACAAACGCTCCCGAT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	1239-1264 bp	1.0	89.5
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1258-1283 bp	1.0	89.3
trnK-UUU-rps16_p3	CTCGTTGCTTTTCGTTTCCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	1444-1490 bp	1.0	88.1
trnK-UUU-rps16_p4	TCTTACAAACGCTCCCGATG	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	1238-1263 bp	0.857143	83.1
trnK-UUU-rps16_p5	TCTTACAAACGCTCCCGATG	CAAAAAGGGGTGTGTTGCTG	50.0	50.0	58.0	58.1	1850-1874 bp	0.857143	80.0