primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GTCTTACAAACGCTCCCGAT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.030311	4329	5384	1239-1264	1.000000	89.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	58.0	58.0	0.0	7.6	0.040076	4329	5384	1258-1283	1.000000	89.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CTCGTTGCTTTTCGTTTCCC	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.056754	4329	5384	1444-1490	1.000000	88.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTTACAAACGCTCCCGATG	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.028923	4329	5384	1238-1263	0.857143	83.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCTTACAAACGCTCCCGATG	CAAAAAGGGGTGTGTTGCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.065957	4329	5384	1850-1874	0.857143	80.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCGACGCTTTAATCCACTCA	AACTGAAGGACCCCTTAACT	20	20	50.0	45.0	58.0	55.6	0.0	0.0	2.358270	9462	10407	891-1077	1.000000	67.0
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	AACTGAAGGACCCCTTAACT	20	20	50.0	45.0	58.2	55.6	0.0	0.0	2.505036	9462	10407	949-1135	1.000000	65.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CCGACGCTTTAATCCACTCA	AAACTGAAGGACCCCTTAACT	20	21	50.0	42.9	58.0	56.5	0.0	0.0	2.548347	9462	10407	892-1078	1.000000	65.5
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGCTTTAATCCACTCAGCC	AACTGAAGGACCCCTTAACT	20	20	50.0	45.0	58.3	55.6	0.0	0.0	2.614621	9462	10407	888-1074	1.000000	65.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CGCTTTAATCCACTCAGCCA	AACTGAAGGACCCCTTAACT	20	20	50.0	45.0	58.3	55.6	0.0	0.0	2.615850	9462	10407	887-1073	1.000000	64.9
atpF-atpH_p1	atpF-atpH	1	CCAGTGAACCAAGGAAACGA	CCTGAGGCAGAGGGAAAAAT	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.559753	14488	15003	643-693	1.000000	81.9
atpF-atpH_p2	atpF-atpH	2	CAAATGGCCAGTGAACCAAG	CCTGAGGCAGAGGGAAAAAT	20	20	50.0	50.0	57.6	57.5	13.0	0.0	0.965434	14488	15003	650-700	1.000000	78.6
atpF-atpH_p3	atpF-atpH	3	CCAGTGAACCAAGGAAACGA	TGGACTGGTTGTAGCATTAGC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.052311	14488	15003	572-622	1.000000	78.2
atpF-atpH_p4	atpF-atpH	4	CCAGTGAACCAAGGAAACGA	GCAGAGGGAAAAATACGAGGT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.050974	14488	15003	637-687	1.000000	78.0
atpF-atpH_p5	atpF-atpH	5	CCAGTGAACCAAGGAAACGA	TGAGGCAGAGGGAAAAATACG	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.052311	14488	15003	641-691	1.000000	78.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	GCATTTCCATCTCCGAGCAT	TTGTTAATCAGGCGACACCC	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.581615	28152	29385	1329-1342	1.000000	77.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GCATTTCCATCTCCGAGCAT	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	58.4	0.0	14.6	0.753180	28152	29385	1293-1306	1.000000	75.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTTCCGCATTTCCATCTCCG	TTGTTAATCAGGCGACACCC	20	20	50.0	50.0	58.6	58.2	0.0	0.0	0.805395	28152	29385	1334-1347	1.000000	75.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GCATTTCCATCTCCGAGCAT	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.873932	28152	29385	1318-1331	1.000000	74.5
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GCATTTCCATCTCCGAGCAT	TAATCAGGCGACACCCAGAT	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.901630	28152	29385	1325-1338	1.000000	74.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	ACAAAACAAGCAAAGGGGGA	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.278576	29457	30199	850-888	1.000000	82.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTGTATCCTTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	58.2	58.2	14.3	0.0	0.305342	29457	30199	815-853	1.000000	82.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	29457	30199	779-817	1.000000	80.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	ACAAAACAAGCAAAGGGGGA	AGAGTCCACTTCTTCCCCAT	20	20	45.0	50.0	58.1	57.6	0.0	0.0	0.478090	29457	30199	911-949	1.000000	80.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTGTATCCTTTTGGCGGCAT	AGAGTCCACTTCTTCCCCAT	20	20	45.0	50.0	58.2	57.6	14.3	0.0	0.504856	29457	30199	876-914	1.000000	80.4
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCCACGGCTGAAACGTATC	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.066573	50959	51532	617-748	1.000000	86.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	50959	51532	585-716	1.000000	86.5
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATCCACGGCTGAAACGTATC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.115963	50959	51532	581-712	1.000000	86.3
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	50959	51532	549-680	1.000000	86.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CTGGTAGAGCAGAGGACTGA	ATCCACGGCTGAAACGTATC	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.200366	50959	51532	605-736	1.000000	85.5
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	69261	69453	255-267	1.000000	84.7
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	69261	69453	253-265	1.000000	84.7
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	69261	69453	256-268	1.000000	84.6
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	69261	69453	266-278	1.000000	84.5
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	69261	69453	267-279	1.000000	84.5
clpP_p1	clpP	1	TCGTGTATTTGCGTGAAATCG	ACGCTTCCACATCAAAGTGA	21	20	42.9	45.0	57.6	57.7	0.0	3.0	1.616827	72930	74991	2151-2159	1.000000	68.3
clpP_p2	clpP	2	TGGAAATCGTGTATTTGCGTG	ACGCTTCCACATCAAAGTGA	21	20	42.9	45.0	57.5	57.7	0.0	3.0	1.718079	72930	74991	2157-2165	1.000000	67.5
clpP_p3	clpP	3	GGAAATCGTGTATTTGCGTGA	ACGCTTCCACATCAAAGTGA	21	20	42.9	45.0	57.3	57.7	0.0	3.0	1.987569	72930	74991	2156-2164	1.000000	65.4
clpP_p4	clpP	4	CGTGTATTTGCGTGAAATCG	ACGCTTCCACATCAAAGTGA	20	20	45.0	45.0	56.2	57.7	0.0	3.0	2.080671	72930	74991	2150-2158	1.000000	64.6
clpP_p5	clpP	5	TGGAAATCGTGTATTTGCGT	ACGCTTCCACATCAAAGTGA	20	20	40.0	45.0	56.1	57.7	0.0	3.0	2.189770	72930	74991	2157-2165	1.000000	63.7
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	74992	75429	509-534	1.000000	83.1
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	CAAACGACCGGGATCATTCA	20	20	50.0	50.0	58.0	58.3	0.0	6.3	0.268011	74992	75429	546-571	1.000000	82.8
clpP-psbB_p3	clpP-psbB	3	GACAGGTCGCACTATACGTC	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.339767	74992	75429	516-541	1.000000	82.3
clpP-psbB_p4	clpP-psbB	4	CTGACAGGTCGCACTATACG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.344042	74992	75429	518-543	1.000000	82.3
clpP-psbB_p5	clpP-psbB	5	GACAGGTCGCACTATACGTC	CAAACGACCGGGATCATTCA	20	20	55.0	50.0	57.9	58.3	0.0	6.3	0.369534	74992	75429	553-578	1.000000	82.0
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	TAGGGTACTTCCGGGAACAG	ATGAGATAGAACCTCCCGCA	20	20	55.0	50.0	58.1	57.9	5.1	0.0	0.231475	111653	112260	813-815	1.000000	79.1
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	TAGGGTACTTCCGGGAACAG	TGGGTTGAGAACGGGAATTG	20	20	55.0	50.0	58.1	58.1	5.1	0.0	0.239084	111653	112260	839-841	1.000000	79.0
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	TAGGGTACTTCCGGGAACAG	AGATAGAACCTCCCGCAGTT	20	20	55.0	50.0	58.1	58.1	5.1	0.0	0.276008	111653	112260	810-812	1.000000	78.8
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	TAGGGTACTTCCGGGAACAG	GCTGTTAACTGACTGGTCGT	20	20	55.0	50.0	58.1	57.8	5.1	15.9	0.293768	111653	112260	762-764	1.000000	78.7
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	TAGGGTACTTCCGGGAACAG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	58.1	58.2	5.1	18.9	0.300003	111653	112260	749-751	1.000000	78.7
ndhF_p1	ndhF	1	GCAGAATACCGTCACCGATT	AGAGCCGGTGAATCAGAAAC	20	20	50.0	50.0	58.1	57.9	0.0	1.9	0.164672	113666	115900	2560-2607	1.000000	80.6
ndhF_p2	ndhF	2	CCTCGGAAAGGCAAGAAAGT	AGAGCCGGTGAATCAGAAAC	20	20	50.0	50.0	58.1	57.9	0.0	1.9	0.203045	113666	115900	2518-2565	1.000000	80.3
ndhF_p3	ndhF	3	GCAGAATACCGTCACCGATT	GAGCCGGTGAATCAGAAACA	20	20	50.0	50.0	58.1	58.2	0.0	1.9	0.255657	113666	115900	2559-2606	1.000000	79.8
ndhF_p4	ndhF	4	AGGGGGACTAAACAGGAACA	AGAGCCGGTGAATCAGAAAC	20	20	50.0	50.0	57.9	57.9	0.0	1.9	0.228256	113666	115900	2464-2512	0.857143	73.6
ndhF_p5	ndhF	5	GTCGAAAGAGAGGGGGACTA	AGAGCCGGTGAATCAGAAAC	20	20	55.0	50.0	57.9	57.9	0.0	1.9	0.241443	113666	115900	2474-2474	0.714286	67.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCGTTGAAAGCTTTTTCGT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.172709	117155	118388	1232-1364	1.000000	86.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCGTTGAAAGCTTTTTCGT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.174080	117155	118388	1233-1365	1.000000	86.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCGTTGAAAGCTTTTTCGT	TCGAACCGAGATGCTTTAGC	20	20	45.0	50.0	58.2	58.1	14.0	0.0	0.242309	117155	118388	1268-1400	1.000000	85.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCGTTGAAAGCTTTTTCGT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	14.0	0.0	0.289156	117155	118388	1242-1374	1.000000	85.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCGTTGAAAGCTTTTTCGT	TCCAATCGAAACGACCGAAA	20	20	45.0	45.0	58.2	57.8	14.0	0.0	0.316760	117155	118388	1457-1583	1.000000	85.2
ccsA-ndhD_p1	ccsA-ndhD	1	TGTGGCTTCTATGGGCTTTC	CGTGGACAAAGTCGAAGCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.189096	119550	119882	444-476	1.000000	89.7
ccsA-ndhD_p2	ccsA-ndhD	2	TGTGGCTTCTATGGGCTTTC	ATCCGTGGACAAAGTCGAAG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.209057	119550	119882	447-479	1.000000	89.5
ccsA-ndhD_p3	ccsA-ndhD	3	TTCCGCAATTGTGGCTTCTA	CGTGGACAAAGTCGAAGCTA	20	20	45.0	50.0	57.8	57.9	29.6	0.0	0.252989	119550	119882	453-485	1.000000	89.0
ccsA-ndhD_p4	ccsA-ndhD	4	TTCCGCAATTGTGGCTTCTA	ATCCGTGGACAAAGTCGAAG	20	20	45.0	50.0	57.8	57.9	29.6	0.0	0.272950	119550	119882	456-488	1.000000	88.8
ccsA-ndhD_p5	ccsA-ndhD	5	AATTCCGCAATTGTGGCTTC	CGTGGACAAAGTCGAAGCTA	20	20	45.0	50.0	57.6	57.9	29.6	0.0	0.428980	119550	119882	455-487	1.000000	87.6
ndhD_p1	ndhD	1	AAAGGCGTCTGGTTCCAATT	AAACAACTCGAAGCATGGGT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.023367	119883	121244	1854-1890	1.000000	82.6
ndhD_p2	ndhD	2	AAAGGCGTCTGGTTCCAATT	TCTGCTCCAAGAACAGAGGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.020080	119883	121244	1948-1984	1.000000	82.5
ndhD_p3	ndhD	3	AAAGGCGTCTGGTTCCAATT	GGCATGAAACAACTCGAAGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.035520	119883	121244	1860-1896	1.000000	82.5
ndhD_p4	ndhD	4	AAAGGCGTCTGGTTCCAATT	CTGCTCCAAGAACAGAGGAC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.096476	119883	121244	1947-1983	1.000000	81.9
ndhD_p5	ndhD	5	AAAGGCGTCTGGTTCCAATT	TGCTTCTGCTCCAAGAACAG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.116409	119883	121244	1952-1988	1.000000	81.7
ycf1_p1	ycf1	1	TGCGGGCGATATTTTTAGCT	ACTCCCAACGCTCAAACAAT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.056950	131404	131523	667-769	1.000000	89.8
ycf1_p2	ycf1	2	ACCACTCGGCAACTTGAAAT	ACTCCCAACGCTCAAACAAT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.037487	131404	131523	694-796	1.000000	89.5
ycf1_p3	ycf1	3	CGTTTTAGGGGTGGACCAAA	ACTCCCAACGCTCAAACAAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034678	131404	131523	959-1061	1.000000	86.9
ycf1_p4	ycf1	4	CGTTTTAGGGGTGGACCAAA	TTCAACTCCCAACGCTCAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061496	131404	131523	963-1065	1.000000	86.7
ycf1_p5	ycf1	5	CGTTTTAGGGGTGGACCAAA	TTTCAACTCCCAACGCTCAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061496	131404	131523	964-1066	1.000000	86.7
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	ATGAGATAGAACCTCCCGCA	TAGGGTACTTCCGGGAACAG	20	20	50.0	55.0	57.9	58.1	0.0	5.1	0.231475	133799	134406	813-815	1.000000	79.1
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	TGGGTTGAGAACGGGAATTG	TAGGGTACTTCCGGGAACAG	20	20	50.0	55.0	58.1	58.1	0.0	5.1	0.239084	133799	134406	839-841	1.000000	79.0
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	AGATAGAACCTCCCGCAGTT	TAGGGTACTTCCGGGAACAG	20	20	50.0	55.0	58.1	58.1	0.0	5.1	0.276008	133799	134406	810-812	1.000000	78.8
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	GCTGTTAACTGACTGGTCGT	TAGGGTACTTCCGGGAACAG	20	20	50.0	55.0	57.8	58.1	15.9	5.1	0.293768	133799	134406	762-764	1.000000	78.7
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TGGTCGTAGGTTCGAATCCT	TAGGGTACTTCCGGGAACAG	20	20	50.0	55.0	58.2	58.1	18.9	5.1	0.300003	133799	134406	749-751	1.000000	78.7