Hotspot

ycf58

Genome · 66239-66673 · 435 bp

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Pi 0.272

MarkerSeek score 75.60

Primer available yes

Label ycf58

Feature Metadata

label_name: ycf58
feature_type: gene
region: Genome
length_bp: 435
variable_sites: 118
pi: 0.272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf58_p1	TTTCTTCGAAACGCTGCAAG	TCAGTGGACATGGAATATTTCAGA	45.0	37.5	57.6	57.7	1627 bp	0.5	22.4
ycf58_p2	TTCTTCGAAACGCTGCAAGT	TCAGTGGACATGGAATATTTCAGA	45.0	37.5	58.7	57.7	1626 bp	0.5	20.0
ycf58_p3	TTTCTTCGAAACGCTGCAAG	CAGTGGACATGGAATATTTCAGA	45.0	39.1	57.6	56.3	1626 bp	0.5	20.0
ycf58_p4	TTTCTTCGAAACGCTGCAAG	TCAGTGGACATGGAATATTTCAG	45.0	39.1	57.6	56.3	1627 bp	0.5	20.0
ycf58_p5	TTTCTTCGAAACGCTGCAAG	TTCAGGCAAAAATGCAAAAA	45.0	30.0	57.6	53.3	1702 bp	0.5	20.0