Hotspot

tilS

Genome · 31295-32278 · 984 bp

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Pi 0.204

MarkerSeek score 80.20

Primer available yes

Label tilS

Feature Metadata

label_name: tilS
feature_type: gene
region: Genome
length_bp: 984
variable_sites: 198
pi: 0.204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tilS_p1	TGGTGCTCCTTTATGTCCTTG	CCATTGAGTTAGCAGCCCTT	47.6	50.0	57.9	57.9	1570-1581 bp	1.0	79.4
tilS_p2	TGGTGCTCCTTTATGTCCTTG	CTCGAACCCGGAACTAATCG	47.6	55.0	57.9	58.2	1610-1621 bp	1.0	79.2
tilS_p3	GGTGCTCCTTTATGTCCTTGT	CCATTGAGTTAGCAGCCCTT	47.6	50.0	57.9	57.9	1569 bp	0.5	50.3
tilS_p4	TGGTGCTCCTTTATGTCCTTG	ACAACCTGGTGCTTTTGACA	47.6	45.0	57.9	58.2	2185 bp	0.5	50.2
tilS_p5	GGTGCTCCTTTATGTCCTTGT	ACAACCTGGTGCTTTTGACA	47.6	45.0	57.9	58.2	2184 bp	0.5	50.2