Hotspot

secY

Genome · 126057-127292 · 1236 bp

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Pi 0.156

MarkerSeek score 79.60

Primer available yes

Label secY

Feature Metadata

label_name: secY
feature_type: gene
region: Genome
length_bp: 1236
variable_sites: 191
pi: 0.156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
secY_p1	AACATCAGGAGCAGCCAAAA	TCAGTACCTGCGATTCTTGC	45.0	50.0	58.0	58.0	1699 bp	0.5	59.9
secY_p2	ACATCAGGAGCAGCCAAAAT	TCAGTACCTGCGATTCTTGC	45.0	50.0	57.8	58.0	1698 bp	0.5	58.1
secY_p3	CCTTCTGCAACAGGATCAGG	TCAGTACCTGCGATTCTTGC	55.0	50.0	58.3	58.0	1668 bp	0.5	57.9
secY_p4	AACATCAGGAGCAGCCAAAA	GTACCTGCGATTCTTGCCAT	45.0	50.0	58.0	58.3	1696 bp	0.5	57.3
secY_p5	TCTGCAACAGGATCAGGAGT	TCAGTACCTGCGATTCTTGC	50.0	50.0	58.4	58.0	1665 bp	0.5	57.1